Recombinant Anti-Calnexin antibody [EPR21205] (ab213243)
![Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)](https://www.abcam.com/ps/products/213/ab213243/Images/ab213243-289254-anti-calnexin-antibody-epr21205-western-blot.jpg "Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21205] to Calnexin
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Calnexin antibody [EPR21205]
See all Calnexin primary antibodies -
Description
Rabbit monoclonal [EPR21205] to Calnexin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse Calnexin. Expressed in HEK293 Cells
Database link: NP_031623.1 -
Positive control
- WB: NIH3T3, HeLa, HAP1 ICC-IF: HeLa and NIH3T3 cells.
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21205 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
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Matched antibody pairs
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab213243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.05 µg/ml. | |
| WB | Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)
Lane 1: NIH3T3 whole cell lysate (10 µg)
Lane 2: Mouse brain whole tissue lysate (10 µg)
Lane 3: HeLa whole cell lysate (10 µg)
Lane 4: Hap1 Wild-type whole cell lysate (10 µg)
Lane 5: CANX knockout Hap1 whole cell lysate (10 µg)Lanes 1 - 5: Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1µg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)](https://www.abcam.com/ps/products/213/ab213243/Images/ab213243-291234-anti-calnexin-antibody-epr21205-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)ab213243 staining Calnexin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)](https://www.abcam.com/ps/products/213/ab213243/Images/ab213243-291233-anti-calnexin-antibody-epr21205-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)ab213243 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Anti-Calnexin antibody [EPR21205] (ab213243)](https://www.abcam.com/ps/products/213/ab213243/Images/ab213243-4-benefits-of-recombinant-antibodies.png)
Anti-Calnexin antibody [EPR21205] (ab213243)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab213243 has been referenced in 2 publications.
- Wang X et al. Exosome-delivered circRNA promotes glycolysis to induce chemoresistance through the miR-122-PKM2 axis in colorectal cancer. Mol Oncol 14:539-555 (2020). PubMed: 31901148
- Xue J et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
