Validated using a knockout cell line
Recombinant

Recombinant Anti-Calnexin antibody [EPR21205] (ab213243)

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Overview

  • Product name

    Anti-Calnexin antibody [EPR21205]
    See all Calnexin primary antibodies

  • Description

    Rabbit monoclonal [EPR21205] to Calnexin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Mouse Calnexin. Expressed in HEK293 Cells
    Database link: NP_031623.1

  • Positive control

    • WB: NIH3T3, HeLa, HAP1 ICC-IF: HeLa and NIH3T3 cells.

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21205

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.05 µg/ml.
WB Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa.

Target

  • Function

    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

  • Sequence similarities

    Belongs to the calreticulin family.

  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • Calnexin antibody
      • CALX_HUMAN antibody
      • CANX antibody
      • CNX antibody
      • FLJ26570 antibody
      • Histocompatibility complex class I antigen binding protein p88 antibody
      • IP90 antibody
      • Major histocompatibility complex class I antigen-binding protein p88 antibody
      • p90 antibody
      see all

    Images

    • Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)
      Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)

      Lane 1: NIH3T3 whole cell lysate (10 µg)
      Lane 2: Mouse brain whole tissue lysate (10 µg)
      Lane 3: HeLa whole cell lysate (10 µg)
      Lane 4: Hap1 Wild-type whole cell lysate (10 µg)
      Lane 5: CANX knockout Hap1 whole cell lysate (10 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1µg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)
      Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)

      ab213243 staining Calnexin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)
      Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)

      ab213243 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References

    ab213243 has not yet been referenced specifically in any publications.

    Publishing research using ab213243? Please let us know so that we can cite the reference in this datasheet.

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