Overview

  • Product name
    Anti-Calnexin - ER membrane marker antibody - ER Marker
    See all Calnexin primary antibodies
  • Description
    Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker
  • Host species
    Rabbit
  • Specificity
    Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WB, IP, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey, Quail
  • Immunogen

    Synthetic peptide:

    (C)EEDEILNRSPRNRKPRRE

    conjugated to KLH, corresponding to C terminal amino acids 575-592 of Dog Calnexin. This sequence is identical to mouse, human and rat calnexin over these residues.

  • Positive control
    • Heat Shocked HeLa Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
ICC/IF
IHC-FoFr
WB
IP
Flow Cyt
ICC
  • Application notes
    Flow Cyt: 1/100.
    ICC: 1/200.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-FoFr: Use at an assay dependent dilution (PMID 18621691).
    IP: 1/100.
    WB: 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

    • Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin’s Fixative Solution. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.

    • All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution

      All lanes : Mixture of rat tissue lysates

      Predicted band size: 90 kDa

    • Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody: FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /

    • Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.

    References

    This product has been referenced in:
    • Aryal B  et al. Absence of ANGPTL4 in adipose tissue improves glucose tolerance and attenuates atherogenesis. JCI Insight 3:N/A (2018). Read more (PubMed: 29563332) »
    • Andersen S  et al. The N-terminally truncated µ3 and µ3-like opioid receptors are transcribed from a novel promoter upstream of exon 2 in the human OPRM1 gene. PLoS One 8:e71024 (2013). ICC/IF ; Human . Read more (PubMed: 23951073) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - other (Subcellular fractionation)
    Loading amount
    12 µg
    Specification
    Subcellular fractionation
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 03 2009

    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
    Loading amount
    10 µg
    Specification
    meiotic egg extract
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Nov 19 2008

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Fruit fly (Drosophila melanogaster) Cell (eye disc)
    Specification
    eye disc
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Apr 30 2008

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    50 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Dr. Ying Chen

    Verified customer

    Submitted Aug 03 2007

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (kidney)
    Specification
    kidney
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 20 2007

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary cortical neuron)
    Specification
    Primary cortical neuron
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    Submitted May 14 2007

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SH-SY5Y (Neuroblastoma))
    Specification
    SH-SY5Y (Neuroblastoma)
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    Submitted Mar 20 2007

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa- cervical carcinoma)
    Specification
    HeLa- cervical carcinoma
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

    Dr. Christopher Cornell

    Verified customer

    Submitted Apr 18 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up