Western blot abreview for Anti-Calnexin - ER membrane marker antibody - ER Marker

Excellent
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
10 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA / PBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 105 kDa Non-specific: 80 kDa

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Submitted Nov 19 2008

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