Western blot abreview for Anti-Calnexin - ER membrane marker antibody - ER Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Subcellular fractionation)
Loading amount
12 µg
Specification
Subcellular fractionation
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% blotto

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG HRP conjugated
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 90 kDa
Positive control
ER fraction

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Submitted Sep 03 2009

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