Immunocytochemistry/ Immunofluorescence abreview for Anti-Calnexin - ER membrane marker antibody - ER Marker

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y (Neuroblastoma))
Specification
SH-SY5Y (Neuroblastoma)
Fixative
Methanol
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Fixed with ice cold methonal for 10 minutes.
Works also with 4% PFA as fixative.
Cells were permeabilized for 10 minutes with 0,5% saponin in either case.
The primary AB was incubated overnight at 4°C.

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Submitted Mar 20 2007

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