Immunocytochemistry/ Immunofluorescence abreview for Anti-Calnexin - ER membrane marker antibody - ER Marker

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary cortical neuron)
Specification
Primary cortical neuron
Fixative
Methanol
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Blocked with 10% serum in 1% BSA. Fixed with ice-cold methanol for 10 minutes. Primary AB was incubated overnight at 4°C.

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Submitted May 14 2007

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