Western blot abreview for Anti-Calnexin - ER membrane marker antibody - ER Marker

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IGg
Host species: Rat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
supersignal PICO
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~105 kDa Non-specific: ~40, 45, 70 kDa

Additional data

Abcam response
Thank you for your feedback. I am unsure in this instance whether endogenous peroxidases present in liver samples were blocked and this may account for some of the non specific bands that have been observed.

Dr. Ying Chen

Verified customer

Submitted Aug 03 2007

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