Anti-Calnexin (phospho S583) antibody (ab58503)
Key features and details
- Rabbit polyclonal to Calnexin (phospho S583)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin (phospho S583) antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin (phospho S583) -
Host species
Rabbit -
Specificity
ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic phosphopeptide derived from human Calnexin - ER membrane marker around the phosphorylation site of serine 583 (N-R-SP-P-R).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Rat
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Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35565 Rat
- Unigene: 567968 Human
- Unigene: 1762 Rat
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilution
Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 90 kDa
Observed band size: 90 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab58503 has been referenced in 2 publications.
- Dallavilla T et al. Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin. PLoS Comput Biol 12:e1004774 (2016). PubMed: 26900856
- Zhao Z TGFß and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth Defects Res B Dev Reprod Toxicol 101:364-70 (2014). Mouse . PubMed: 25231192