Anti-Calnexin (phospho S583) antibody (ab58503)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Calnexin (phospho S583) antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin (phospho S583) -
Host species
Rabbit -
Specificity
ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic phosphopeptide derived from human Calnexin - ER membrane marker around the phosphorylation site of serine 583 (N-R-SP-P-R).
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Positive control
- HeLa cell extracts treated with EGF (200ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab58503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 22534324 |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- SwissProt: P35565 Rat
- Unigene: 567968 Human
see all -
Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilution
Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 90 kDa
Observed band size: 90 kDa
Datasheets and documents
References
This product has been referenced in:
- Dallavilla T et al. Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin. PLoS Comput Biol 12:e1004774 (2016). Read more (PubMed: 26900856) »
- Zhao Z TGFß and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth Defects Res B Dev Reprod Toxicol 101:364-70 (2014). Mouse . Read more (PubMed: 25231192) »
