Anti-Calnexin (phospho S583) antibody (ab58503)

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Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)

    Key features and details

    • Rabbit polyclonal to Calnexin (phospho S583)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Calnexin (phospho S583) antibody
      See all Calnexin primary antibodies

    • Description

      Rabbit polyclonal to Calnexin (phospho S583)

    • Host species

      Rabbit

    • Specificity

      ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic phosphopeptide derived from human Calnexin - ER membrane marker around the phosphorylation site of serine 583 (N-R-SP-P-R).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg+2 and Ca+2
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab58503 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

    Target

    • Function

      Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

    • Sequence similarities

      Belongs to the calreticulin family.

    • Cellular localization

      Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      • Alternative names

        • Calnexin antibody
        • CALX_HUMAN antibody
        • CANX antibody
        • CNX antibody
        • FLJ26570 antibody
        • Histocompatibility complex class I antigen binding protein p88 antibody
        • IP90 antibody
        • Major histocompatibility complex class I antigen-binding protein p88 antibody
        • p90 antibody
        see all

      Images

      • Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)
        Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)
        All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilution

        Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
        Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide

        Predicted band size: 90 kDa
        Observed band size: 90 kDa

      References (2)

      Publishing research using ab58503? Please let us know so that we can cite the reference in this datasheet.

      ab58503 has been referenced in 2 publications.

      • Dallavilla T  et al. Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin. PLoS Comput Biol 12:e1004774 (2016). PubMed: 26900856
      • Zhao Z TGFß and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth Defects Res B Dev Reprod Toxicol 101:364-70 (2014). Mouse . PubMed: 25231192

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