Anti-Calpain 1 antibody (ab155677)
Key features and details
- Rabbit polyclonal to Calpain 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calpain 1 antibody
See all Calpain 1 primary antibodies -
Description
Rabbit polyclonal to Calpain 1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB MouseHuman -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 317-493 of Human Calpain 1 (UniProt ID: P07384).
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Positive control
- 293T, A431, Jurkat, Raji and NIH 3T3 whole cell lysates; A431 cells; Human BT474 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Mouse
Human
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All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 82 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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Database links
- Entrez Gene: 823 Human
- Entrez Gene: 12333 Mouse
- Entrez Gene: 29153 Rat
- Omim: 114220 Human
- SwissProt: P07384 Human
- SwissProt: O35350 Mouse
- SwissProt: P97571 Rat
- Unigene: 502842 Human
see all -
Alternative names
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
Images
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Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431 cells labeling Calpain 1 with ab155677 at 1/200 dilution (green). Lower image shows costaining with Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody (ab155677)Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling Calpain 1 with ab155677 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155677 has not yet been referenced specifically in any publications.