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- Specific References
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Overview
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Product nameAnti-Calpain 1 antibody
See all Calpain 1 primary antibodies -
DescriptionRabbit polyclonal to Calpain 1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 317-493 of Human Calpain 1 (UniProt ID: P07384).
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Positive control
- 293T, A431, Jurkat, Raji and NIH 3T3 whole cell lysates; A431 cells; Human BT474 xenograft tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 82 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
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Database links
- Entrez Gene: 823 Human
- Entrez Gene: 12333 Mouse
- Entrez Gene: 29153 Rat
- Omim: 114220 Human
- SwissProt: P07384 Human
- SwissProt: O35350 Mouse
- SwissProt: P97571 Rat
- Unigene: 502842 Human
see all -
Alternative names
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
Images
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Western blot - Anti-Calpain 1 antibody (ab155677)Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Western blot - Anti-Calpain 1 antibody (ab155677)Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (ab155677)Immunofluorescence analysis of methanol-fixed A431 cells labeling Calpain 1 with ab155677 at 1/200 dilution (green). Lower image shows costaining with Hoechst 33342 (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody (ab155677)Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling Calpain 1 with ab155677 at 1/500 dilution.
Protocols
References
ab155677 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"