Anti-Calpain 1 antibody (ab155677)

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Western blot - Anti-Calpain 1 antibody (ab155677)
  • Western blot - Anti-Calpain 1 antibody (ab155677)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (ab155677)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody (ab155677)

Key features and details

  • Rabbit polyclonal to Calpain 1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Calpain 1 antibody
    See all Calpain 1 primary antibodies

  • Description

    Rabbit polyclonal to Calpain 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 317-493 of Human Calpain 1 (UniProt ID: P07384).

  • Positive control

    • 293T, A431, Jurkat, Raji and NIH 3T3 whole cell lysates; A431 cells; Human BT474 xenograft tissue.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
All applications
Rat
Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.

  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain 1, (mu/I) large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain, large polypeptide L1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANP1 antibody
    • CANPL 1 antibody
    • CANPL1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation inducing protein 30 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all

Images

  • Western blot - Anti-Calpain 1 antibody (ab155677)
    Western blot - Anti-Calpain 1 antibody (ab155677)
    Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 82 kDa



    7.5% SDS PAGE
  • Western blot - Anti-Calpain 1 antibody (ab155677)
    Western blot - Anti-Calpain 1 antibody (ab155677)
    Anti-Calpain 1 antibody (ab155677) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 82 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (ab155677)
    Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (ab155677)
    Immunofluorescence analysis of methanol-fixed A431 cells labeling Calpain 1 with ab155677 at 1/200 dilution (green). Lower image shows costaining with Hoechst 33342 (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody (ab155677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody (ab155677)
    Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling Calpain 1 with ab155677 at 1/500 dilution.

References (0)

Publishing research using ab155677? Please let us know so that we can cite the reference in this datasheet.

ab155677 has not yet been referenced specifically in any publications.

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Western blot abreview for Anti-Calpain 1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (muscle)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
50 µg
Specification
muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. Baptiste Jude

Verified customer

Submitted Jul 18 2016

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