Product nameAnti-Calpain 1 antibody
See all Calpain 1 primary antibodies
DescriptionRabbit polyclonal to Calpain 1
Specificityab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.).
Tested applicationsSuitable for: WB, ICC, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Calpain 1.
(Peptide available as
- Calpain 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody has been peptide-affinity purified.
Our Abpromise guarantee covers the use of ab28258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 82 kDa. 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.|
|ICC||Use at an assay dependent concentration.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution + Calpain 1 at 10 µg
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab28258 staining human HUVEC cells by ICC/IF. Cells were PFA fixed and permeablized in 0.5% Triton X-100 prior to blocking in 1% serum for 16 hours at 4°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 25°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/100, was used as the secondary.
ab28258 detecting Calpain 1 - Domain I in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The primary antibody was diluted 1/250 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.
Abbreviations in the figure:
US Unstained, Red Peak;
IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
Calpain: Green Peak.
This product has been referenced in:
- Pahima H et al. Hypoxic-induced truncation of voltage-dependent anion channel 1 is mediated by both asparagine endopeptidase and calpain 1 activities. Oncotarget 9:12825-12841 (2018). Read more (PubMed: 29560113) »
- Zhao WD et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). Read more (PubMed: 29895952) »