• Product name

  • Description

    Rabbit polyclonal to Calpain 1
  • Host species

  • Specificity

    This antibody binds to reduced and non reduced Calpain 1. It reacts with Domain IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. It but does not cross react with the other calpain family members (Calpain 2, calpain 94, ncl 2, ncl 3, etc.).
  • Tested applications

    Suitable for: WB, IHC (PFA fixed), ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide based on Domain IV of the large subunit of Human Calpain 1. (Peptide available as ab41300.)

  • Positive control

    • SW1353 whole cell lysate (ab7915) can be used as a positive control in WB. Cell lysates from human chondrosarcoma.



Our Abpromise guarantee covers the use of ab39170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.
IHC (PFA fixed) Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.


  • Function

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity

  • Sequence similarities

    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain 1, (mu/I) large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain, large polypeptide L1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANP1 antibody
    • CANPL 1 antibody
    • CANPL1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation inducing protein 30 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all


  • ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Telechea-Fernández M  et al. New localization and function of calpain-2 in nucleoli of colorectal cancer cells in ribosomal biogenesis: effect of KRAS status. Oncotarget 9:9100-9113 (2018). Read more (PubMed: 29507677) »
  • Weber JJ  et al. Calpastatin ablation aggravates the molecular phenotype in cell and animal models of Huntington disease. Neuropharmacology 133:94-106 (2018). WB . Read more (PubMed: 29355642) »
See all 20 Publications for this product

Customer reviews and Q&As


I now have some further information to share with you in regards to the anti-Calpain 1 antibody - Domain IV (ab39170).

The immunogen used to raise this antibody is taken from thecarboxyterminal area of Calpain-1. As proteolytic processing of Calpain-1 occurs at the amino end, the antibody is likely to be able to detect both thezymogen and cleaved enzyme. It is therefore possible that you have been picking up both forms in your Western blot.

You may be interested to know that we have the blocking peptide for this antibody available under the catalogue number https://www.abcam.com/index.html?datasheet=41300.

I hope this information has been of help. If you require any further information, please do not hesitate to ask.

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