Recombinant Anti-Calpain 1 antibody [EPR3319] (ab108400)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3319] to Calpain 1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Calpain 1 antibody [EPR3319]
See all Calpain 1 primary antibodies -
Description
Rabbit monoclonal [EPR3319] to Calpain 1 -
Host species
Rabbit -
Specificity
ab108400 recognises the large subunit of Calpain -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, U937, A431, (D) 293T, and T47D cell lysates; Human kidney tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3319 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 82 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
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ICC/IF |
1/50.
For unpurified use at 1/250 - 1/500. |
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Flow Cyt (Intra) |
1/380.
For unpurified use at 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
ICC/IF
1/50. For unpurified use at 1/250 - 1/500. |
Flow Cyt (Intra)
1/380. For unpurified use at 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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Database links
- Entrez Gene: 823 Human
- Entrez Gene: 12333 Mouse
- Entrez Gene: 29153 Rat
- Omim: 114220 Human
- SwissProt: P07384 Human
- SwissProt: O35350 Mouse
- SwissProt: P97571 Rat
- Unigene: 502842 Human
see all -
Alternative names
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
Images
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All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400)
Lane 1 : Wild type HAP1 whole cell lysate
Lane 2 : CAPN1 (Calpain 1) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Predicted band size: 82 kDaAb108400 was shown to recognize Calpain 1 in wild-type HAP1. A faint cross-reactive band was observed in CAPN1 (Calpain 1) knockout cells at 82 kDa when over-exposed. Due to the immunogen’s homology with CAPN2 (Calpain 2 Uniprot: P17655), this band could correspond to the Calpain 2 protein. Please note that cross-reactivity with this protein has not been confirmed experimentally.
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Overlay histogram showingJurkat cells stained with ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
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ab108400 staining Calpain 1 in the T-47D cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/70). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)
ab108400 staining Calpain 1 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/200). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 82 kDa -
Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution + C6 Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 82 kDa -
Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + T47-D Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 82 kDa -
Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa -
All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : T47D cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 82 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)
ab108400, unpurified, at 1/500 dilution staining Calpain in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
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ab108400, unpurified, at 1/250 dilution staining Calpain in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab108400 has been referenced in 14 publications.
- Wang X et al. Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis. J Cell Mol Med 25:2238-2253 (2021). PubMed: 33439514
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- He J et al. Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis. Braz J Med Biol Res 54:e10498 (2021). PubMed: 33656055
- Xu J et al. Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3ß) in Neurons. Ann Transplant 25:e924093 (2020). PubMed: 32686658
- Kudryashev JA et al. An Activity-Based Nanosensor for Traumatic Brain Injury. ACS Sens 5:686-692 (2020). PubMed: 32100994