Overview

  • Product name
    Calpain 1 Inhibitor Screening Kit
    See all Calpain 1 kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Calpain 1 Inhibitor Screening Kit (ab155888) is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain 1 inhibitor ) is included to compare the efficacy of test inhibitors.

  • Notes

    Calpain 1 is a calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Calpain 1 Assay Buffer WM 1 x 25ml
    Calpain 1 Enzyme Green 1 x 0.2ml
    Calpain 1 Inhibitor Control Blue 1 x 0.5ml
    Calpain 1 Substrate Red 1 x 0.2ml
  • Research areas
  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Alternative names
    • Ca2 activated neutral protease
    • Calcium activated neutral proteinase
    • Calcium activated neutral proteinase small subunit
    • Calcium dependent protease small subunit
    • Calcium dependent protease small subunit 1
    • Calcium-activated neutral proteinase 1
    • Calpain 1
    • Calpain 1 large subunit
    • Calpain 1, (mu/I) large subunit
    • Calpain mu type
    • Calpain mu-type
    • Calpain regulatory subunit
    • Calpain small subunit 1
    • Calpain, large polypeptide L1
    • Calpain-1 catalytic subunit
    • Calpain-1 large subunit
    • CAN1_HUMAN
    • CANP
    • CANP 1
    • CANP small subunit
    • CANP1
    • CANPL 1
    • CANPL1
    • CAPN 1
    • CAPN1
    • Cell proliferation inducing protein 30
    • Cell proliferation-inducing gene 30 protein
    • Micromolar Calpain
    • Micromolar-calpain
    • Mu Calpain
    • muCANP
    • muCL
    see all

Associated products

Images

Protocols

References

ab155888 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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