Overview

  • Product name

    Anti-Calpain 11 antibody
  • Description

    Rabbit polyclonal to Calpain 11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 61-318 of Human Calpain 11.

  • Positive control

    • NT2D1 and HeLa whole cell lysates; SCM1 xenograft; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.
  • Cellular localization

    Cytoplasmic vesicle; secretory vesicle; acrosome
  • Database links

  • Alternative names

    • Calcium activated neutral proteinase 11 antibody
    • Calpain11 antibody
    • CAPN 11 antibody
    • CAPN11 antibody

Images

  • All lanes : Anti-Calpain 11 antibody (ab151324) at 1/5000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 81 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SCM1 xenograft labeling Calpain 11 with ab151324 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Calpain 11 with ab151324 at 1/200 dilution (green). Alpha-tubulin filaments labeled in red.

References

ab151324 has not yet been referenced specifically in any publications.

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