Overview

  • Product name
    Anti-Calpain 15 antibody [EPR11078]
    See all Calpain 15 primary antibodies
  • Description
    Rabbit monoclonal [EPR11078] to Calpain 15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Calpain 15 aa 1-100. The exact sequence is proprietary.
    Database link: O75808

  • Positive control
    • Human fetal brain, Human fetal lung, HeLa, 293T and SH-SY5Y lysates; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 117 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Tissue specificity
      Widely expressed with higher expression in brain.
    • Sequence similarities
      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 5 RanBP2-type zinc fingers.
    • Information by UniProt
    • Database links
    • Alternative names
      • calpain 15 antibody
      • Calpain-15 antibody
      • CAN15_HUMAN antibody
      • CAPN15 antibody
      • MGC131491 antibody
      • Small optic lobe protein antibody
      • Small optic lobes homolog antibody
      • Solh antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling Calpain 15 with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    • Anti-Calpain 15 antibody [EPR11078] (ab170926) at 1/1000 dilution + SH SY5Y cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 117 kDa

    • All lanes : Anti-Calpain 15 antibody [EPR11078] (ab170926) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 117 kDa

    References

    ab170926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170926.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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