Overview

  • Product name

    Anti-Calpain 15/SOLH antibody [EPR11078]
    See all Calpain 15/SOLH primary antibodies
  • Description

    Rabbit monoclonal [EPR11078] to Calpain 15/SOLH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Calpain 15/SOLH aa 1-100. The exact sequence is proprietary.
    Database link: O75808

  • Positive control

    • Human fetal brain, Human fetal lung, HeLa, 293T and SH-SY5Y lysates; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Calpain 15

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 117 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Tissue specificity

      Widely expressed with higher expression in brain.
    • Sequence similarities

      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 5 RanBP2-type zinc fingers.
    • Information by UniProt
    • Database links

    • Alternative names

      • calpain 15 antibody
      • Calpain-15 antibody
      • CAN15_HUMAN antibody
      • CAPN15 antibody
      • MGC131491 antibody
      • Small optic lobe protein antibody
      • Small optic lobes homolog antibody
      • Solh antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling Calpain 15/SOLH with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    • Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution + SH SY5Y cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 117 kDa

    • All lanes : Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 117 kDa

    References

    ab170926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170926.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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