Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11078] to Calpain 15/SOLH
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-Calpain 15/SOLH antibody [EPR11078]
See all Calpain 15/SOLH primary antibodies
DescriptionRabbit monoclonal [EPR11078] to Calpain 15/SOLH
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Calpain 15/SOLH aa 1-100. The exact sequence is proprietary.
Database link: O75808
- Human fetal brain, Human fetal lung, HeLa, 293T and SH-SY5Y lysates; 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Calpain 15
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 117 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityWidely expressed with higher expression in brain.
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 5 RanBP2-type zinc fingers.
- Information by UniProt
- calpain 15 antibody
- Calpain-15 antibody
- CAN15_HUMAN antibody
Flow cytometric analysis of permeabilized 293T cells labeling Calpain 15/SOLH with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution + SH SY5Y cell lysate at 10 µg
Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 117 kDa
All lanes : Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 117 kDa
ab170926 has not yet been referenced specifically in any publications.