Anti-Calpain 2 antibody [1E8] (ab713)

Reviews (1)Q&A (1)

Key features and details

  • Mouse monoclonal [1E8] to Calpain 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Calpain 2 antibody [1E8]
    See all Calpain 2 primary antibodies

  • Description

    Mouse monoclonal [1E8] to Calpain 2

  • Host species

    Mouse

  • Specificity

    Recognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Cow, Human

  • Immunogen

    Bovine cardiac Calpain 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 µg/ml. Fix with 2% paraformaldehyde.
WB Use a concentration of 1 µg/ml.

Target

  • Function

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.

  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt

  • Database links

    • Alternative names

      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Images

    • Western blot - Anti-Calpain 2 antibody [1E8] (ab713)
      Western blot - Anti-Calpain 2 antibody [1E8] (ab713)

      Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.

    • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody [1E8] (ab713)
      Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody [1E8] (ab713)

      Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab713? Please let us know so that we can cite the reference in this datasheet.

    ab713 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    The vial received on order 236762 was contaminated and experiments in WB and ICC did not work.

    Read More
    Answer

    I am sorry that the vial you received was contaminated and can confirm that a replacement vial will be sent to you this afternoon on order 241265. If you still experience problems with the new vial please provide your protocol details via the links below and we will look into this matter and see if we can provide suggestions to improve the results. If the problem is antibody related we will be happy to offer a refund on your original order if you contact us within 120 days of receipt of the new vial and if the antibody doesn't perform as described on the datasheet. For IHC: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=713&mode=questionaire For WB: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=713&mode=questionaire I would like to also encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calpain 2 antibody [1E8]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (chondrocytes and fibrochondrocytes)
    Specification
    chondrocytes and fibrochondrocytes
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2%
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 09 2006

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