Anti-Calpain 2 antibody [1E8] (ab713)
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Overview
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Product nameAnti-Calpain 2 antibody [1E8]
See all Calpain 2 primary antibodies -
DescriptionMouse monoclonal [1E8] to Calpain 2
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Host speciesMouse
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SpecificityRecognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain
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Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
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Species reactivityReacts with: Cow, Human
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Immunogen
Bovine cardiac Calpain 2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading...
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PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone number1E8
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | Use a concentration of 0.5 µg/ml. Fix with 2% paraformaldehyde. | |
ELISA | Use at an assay dependent dilution. | |
WB | Use a concentration of 1 µg/ml. |
Target
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FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
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Database links
- Entrez Gene: 281662 Cow
- Entrez Gene: 824 Human
- Omim: 114230 Human
- SwissProt: Q27971 Cow
- SwissProt: P17655 Human
- Unigene: 350899 Human
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Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
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Images
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Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.
Immunodetection of m-calpain using MAb 1E8 (1) in Western blot. -
Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).
Datasheets and documents
References
ab713 has not yet been referenced specifically in any publications.