Anti-Calpain 2 antibody [1E8] (ab713)

Reviews (1)Q&A (1)

Overview

  • Product name
    Anti-Calpain 2 antibody [1E8]
    See all Calpain 2 primary antibodies
  • Description
    Mouse monoclonal [1E8] to Calpain 2
  • Host species
    Mouse
  • Specificity
    Recognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Bovine cardiac Calpain 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 µg/ml. Fix with 2% paraformaldehyde.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml.

Target

  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links
    • Alternative names
      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Images

    • Western blot - Anti-Calpain 2 antibody [1E8] (ab713)
      Western blot - Anti-Calpain 2 antibody [1E8] (ab713)

      Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.

      Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.
    • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody [1E8] (ab713)
      Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody [1E8] (ab713)

      Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).

    References

    ab713 has not yet been referenced specifically in any publications.

    Publishing research using ab713? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    The vial received on order 236762 was contaminated and experiments in WB and ICC did not work.

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    Answer

    I am sorry that the vial you received was contaminated and can confirm that a replacement vial will be sent to you this afternoon on order 241265. If you still experience problems with the new vial please provide your protocol details via the links below and we will look into this matter and see if we can provide suggestions to improve the results. If the problem is antibody related we will be happy to offer a refund on your original order if you contact us within 120 days of receipt of the new vial and if the antibody doesn't perform as described on the datasheet. For IHC: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=713&mode=questionaire For WB: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=713&mode=questionaire I would like to also encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calpain 2 antibody [1E8]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (chondrocytes and fibrochondrocytes)
    Specification
    chondrocytes and fibrochondrocytes
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2%
    Read More

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    Verified customer

    Submitted Mar 09 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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