Anti-Calpain 2 antibody (ab227439)
Key features and details
- Rabbit polyclonal to Calpain 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calpain 2 antibody
See all Calpain 2 primary antibodies -
Description
Rabbit polyclonal to Calpain 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Calpain 2 (internal sequence). The exact sequence is proprietary.
Database link: P17655 -
Positive control
- IHC-P: Mouse colon tissue; Cal27 xenograft tissue. WB: HEK-293T whole cell lysate; Mouse brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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Database links
- Entrez Gene: 281662 Cow
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 397393 Pig
- Entrez Gene: 100009092 Rabbit
- Entrez Gene: 29154 Rat
- Entrez Gene: 703047 Rhesus monkey
- Entrez Gene: 100127211 Sheep
see all -
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
Images
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Paraffin-embedded mouse colon tissue stained for Calpain 2 using ab227439 at 1/500 dilution in immunohistochemical analysis.
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Anti-Calpain 2 antibody (ab227439) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 80 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded Cal27 xenograft tissue stained for Calpain 2 using ab227439 at 1/500 dilution in immunohistochemical analysis.
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Anti-Calpain 2 antibody (ab227439) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 80 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227439 has been referenced in 1 publication.
- Hannes S et al. The Smac mimetic BV6 cooperates with STING to induce necroptosis in apoptosis-resistant pancreatic carcinoma cells. Cell Death Dis 12:816 (2021). PubMed: 34462421