Overview

  • Product name

  • Description

    Rabbit polyclonal to Calpain 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Calpain 2 (internal sequence). The exact sequence is proprietary.
    Database link: P17655

  • Positive control

    • IHC-P: Mouse colon tissue; Cal27 xenograft tissue. WB: HEK-293T whole cell lysate; Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.

Target

  • Function

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium activated neutral proteinase 2 antibody
    • Calcium activated neutral proteinase antibody
    • Calcium-activated neutral protease 2, catalytic subunit antibody
    • Calcium-activated neutral proteinase 2 antibody
    • CALP80 antibody
    • Calpain 2 (m/II) large subunit antibody
    • Calpain 2 catalytic subunit antibody
    • Calpain 2 large catalytic subunit antibody
    • Calpain 2 large subunit antibody
    • Calpain 2, large [catalytic] subunit antibody
    • Calpain large polypeptide L2 antibody
    • Calpain M type antibody
    • Calpain M-type antibody
    • Calpain-2 catalytic subunit antibody
    • Calpain-2 large subunit antibody
    • Calpain2 antibody
    • CAN2_HUMAN antibody
    • CANP 2 antibody
    • CANP L2 antibody
    • CANP2 antibody
    • CANPL 2 antibody
    • CANPL2 antibody
    • CANPml antibody
    • Capa2 antibody
    • CAPN 2 antibody
    • CAPN2 antibody
    • FLJ39928 antibody
    • M calpain antibody
    • M calpin antibody
    • M type antibody
    • M-calpain antibody
    • mCANP antibody
    • Millimolar calpain antibody
    • Millimolar-calpain antibody
    see all

Images

  • Paraffin-embedded mouse colon tissue stained for Calpain 2 using ab227439 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Calpain 2 antibody (ab227439) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded Cal27 xenograft tissue stained for Calpain 2 using ab227439 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Calpain 2 antibody (ab227439) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 80 kDa



    7.5% SDS-PAGE gel.

References

ab227439 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227439.
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