Anti-Calpain 2 antibody (ab236650)
Key features and details
- Rabbit polyclonal to Calpain 2
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calpain 2 antibody
See all Calpain 2 primary antibodies -
Description
Rabbit polyclonal to Calpain 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human Calpain 2 aa 1 to the C-terminus.
Database link: P17655 -
Positive control
- WB: Mouse lung lysate; NIH/3T3, Jurkat, A431, HeLa, A549, HepG2 and HEK-293T whole cell lysates. IHC-P: Human tonsil and appendix tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse lung normal tissue lysate - total protein (ab29297)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- A-431 whole cell lysate (ab7909)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 80 kDa.
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ICC/IF |
1/50 - 1/200.
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IP |
1/200 - 1/2000.
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IHC-P |
1/200 - 1/500.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 80 kDa. |
ICC/IF
1/50 - 1/200. |
IP
1/200 - 1/2000. |
IHC-P
1/200 - 1/500. |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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Database links
- Entrez Gene: 281662 Cow
- Entrez Gene: 102122692 Cynomolgus monkey
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: Q27971 Cow
- SwissProt: Q9GLG1 Cynomolgus monkey
see all -
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
Images
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All lanes : Anti-Calpain 2 antibody (ab236650) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 80 kDa -
Anti-Calpain 2 antibody (ab236650) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80 kDa -
Paraffin-embedded human tonsil tissue stained for Calpain 2 using ab236650 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Paraffin-embedded human appendix tissue stained for Calpain 2 using ab236650 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Calpain 2 (green) using ab236650 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
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Calpain 2 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab236650 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab236650 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236650 has not yet been referenced specifically in any publications.