Key features and details
- Mouse monoclonal [OTI7D2] to Calpain 5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Calpain 5 antibody [OTI7D2]
See all Calpain 5 primary antibodies
DescriptionMouse monoclonal [OTI7D2] to Calpain 5
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Calpain 5 aa 274-578. (NP_004046) produced in E.coli.
SEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEF WMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNR GGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLA IGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPT TFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHV LGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKL SQPIT
Database link: O15484
- IHC-P: Human prostate carcinoma and colon tissue.
Clone OTI7D2 (formerly 7D2).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
Tissue specificityExpressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
Involvement in diseaseVitreoretinopathy, neovascular inflammatory
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain.
- Information by UniProt
- ADNIV antibody
- Calpain htra 3 antibody
- Calpain htra-3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236377 has not yet been referenced specifically in any publications.