Key features and details
- Rabbit polyclonal to Calpain 6
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Calpain 6 antibody
DescriptionRabbit polyclonal to Calpain 6
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
An 18 amino acid peptide from near the carboxy terminus of human Calpain 6.
- Rat lung tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity chromatography purified via peptide column.
Our Abpromise guarantee covers the use of ab76974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 75 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionMicrotubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
Tissue specificityExpressed only in placenta.
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain.
Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
- Information by UniProt
- CALP M antibody
- Calpain-6 antibody
- Calpain-like protease X-linked antibody
ab76974 at 2.5µg/ml staining Calpain6 in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Lane 1 : Anti-Calpain 6 antibody (ab76974) at 0.5 µg/ml
Lane 2 : Anti-Calpain 6 antibody (ab76974) at 1 µg/ml
All lanes : rat lung tissue lysate
Predicted band size: 75 kDa
Observed band size: 75 kDa
Immunofluorescence of CAPN6 in Human Lung cells using ab76974 at 20 ug/ml.
ab76974 has been referenced in 1 publication.
- Filali H et al. Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep. Vet Res 44:14 (2013). IHC-P ; Sheep . PubMed: 23497022