Recombinant Anti-Calpain 9 antibody [EPR13422] (ab181178)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13422] to Calpain 9
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Calpain 9 antibody [EPR13422]
See all Calpain 9 primary antibodies -
Description
Rabbit monoclonal [EPR13422] to Calpain 9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human stomach lysate; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13422 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 79 kDa).
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Notes |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 79 kDa). |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease. -
Tissue specificity
Expressed predominantly in stomach. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 10753 Human
- Omim: 606401 Human
- SwissProt: O14815 Human
- Unigene: 498021 Human
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Alternative names
- Calpain-9 antibody
- Calpain9 antibody
- CAN9_HUMAN antibody
see all
Images
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Anti-Calpain 9 antibody [EPR13422] (ab181178) at 1/10000 dilution + Human stomach lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDa -
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling Calpain 9 with ab181178 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181178 has not yet been referenced specifically in any publications.