Key features and details
- Rabbit polyclonal to Calpain small subunit 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calpain small subunit 1 antibody
See all Calpain small subunit 1 primary antibodies
DescriptionRabbit polyclonal to Calpain small subunit 1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Calpain small subunit 1 aa 1-222. The exact sequence is proprietary.
Database link: P04632
- 293T whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 78% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionRegulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Sequence similaritiesContains 5 EF-hand domains.
DomainThe contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
- Information by UniProt
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
Anti-Calpain small subunit 1 antibody (ab155672) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 28 kDa
12% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells (iced-cold; 5 mins) labeling Calpain small subunit 1 with ab155672 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue, labeling Calpain small subunit 1 with ab155672 at 1/100 dilution.
ab155672 has not yet been referenced specifically in any publications.