Overview

  • Product name

    Anti-Calpain small subunit 1 antibody
    See all Calpain small subunit 1 primary antibodies
  • Description

    Rabbit polyclonal to Calpain small subunit 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Calpain small subunit 1 aa 1-222. The exact sequence is proprietary.
    Database link: P04632

  • Positive control

    • 293T whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Sequence similarities

    Contains 5 EF-hand domains.
  • Domain

    The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
    EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • 30K antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase small subunit antibody
    • Calcium-dependent protease small subunit 1 antibody
    • Calcium-dependent protease small subunit antibody
    • Calpain 4 antibody
    • Calpain 4 small subunit (30K) antibody
    • Calpain 4 small subunit antibody
    • Calpain regulatory subunit antibody
    • Calpain S1 antibody
    • Calpain small polypeptide antibody
    • Calpain small subunit 1 antibody
    • Calpain4 antibody
    • CalpainS1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANPS antibody
    • CAPN 4 antibody
    • CAPN S1 antibody
    • CAPN4 antibody
    • CAPNS 1 antibody
    • CAPNS antibody
    • Capns1 antibody
    • CDPS antibody
    • CPNS1_HUMAN antibody
    • CSS 1 antibody
    • CSS1 antibody
    see all

Images

  • Anti-Calpain small subunit 1 antibody (ab155672) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells (iced-cold; 5 mins) labeling Calpain small subunit 1 with ab155672 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue, labeling Calpain small subunit 1 with ab155672 at 1/100 dilution.

References

ab155672 has not yet been referenced specifically in any publications.

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