Product nameAnti-Calpain small subunit 1 antibody
See all Calpain small subunit 1 primary antibodies
DescriptionRabbit polyclonal to Calpain small subunit 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human Calpain small subunit 1 aa 91-268. The exact sequence is proprietary. (With tag; BC000592).
EANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFG IDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTIC SSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMF RAFKSLDKDGTGQIQVNIQEWLQLTMYS
Database link: P04632
- Mouse spleen tissue lysate; K562 and NIH 3T3 cell lysates; Human colon cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/25 - 1/100.|
FunctionRegulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Sequence similaritiesContains 5 EF-hand domains.
DomainThe contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
- Information by UniProt
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
All lanes : Anti-Calpain small subunit 1 antibody (ab182607) at 1/200 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 60 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 28 kDa
Exposure time: 15 seconds
Gel: 12% SDS-PAGE
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Calpain small subunit 1 with ab182607 at 1/15 dilution (left panel). Right panel treated with the immunogen.
ab182607 has not yet been referenced specifically in any publications.