Recombinant
RabMAb

Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356)

Overview

  • Product name
    Anti-Calpain small subunit 1 antibody [EPR3323]
    See all Calpain small subunit 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3323] to Calpain small subunit 1
  • Host species
    Rabbit
  • Specificity
    Detects Calpain small subunit 1.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calpain small subunit 1 aa 50-150. The exact sequence is proprietary.
    Database link: P04632

  • Positive control
    • T47-D, 293T, A431 and Human spleen lysates. Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
Flow Cyt
  • Application notes
    Flow Cyt: 1/100.
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended as these have been shown to give superior staining.

    WB: 1/1000 - 1/2000. Predicted molecular weight: 28 kDa.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Sequence similarities
      Contains 5 EF-hand domains.
    • Domain
      The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
      EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • 30K antibody
      • Calcium activated neutral proteinase antibody
      • Calcium activated neutral proteinase small subunit antibody
      • Calcium dependent protease small subunit 1 antibody
      • Calcium dependent protease small subunit antibody
      • Calcium-activated neutral proteinase small subunit antibody
      • Calcium-dependent protease small subunit 1 antibody
      • Calcium-dependent protease small subunit antibody
      • Calpain 4 antibody
      • Calpain 4 small subunit (30K) antibody
      • Calpain 4 small subunit antibody
      • Calpain regulatory subunit antibody
      • Calpain small polypeptide antibody
      • Calpain small subunit 1 antibody
      • Calpain4 antibody
      • CalpainS1 antibody
      • CANP antibody
      • CANP small subunit antibody
      • CANPS antibody
      • CAPN 4 antibody
      • CAPN S1 antibody
      • CAPN4 antibody
      • CAPNS 1 antibody
      • CAPNS antibody
      • Capns1 antibody
      • CDPS antibody
      • CPNS1_HUMAN antibody
      • CSS 1 antibody
      • CSS1 antibody
      see all

    Images

    • All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 28 kDa

    • ab92356 at 1/100 dilution staining Calpain small subunit 1 in paraffin-embedded Human kidney

    References

    ab92356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92356.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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