Recombinant Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free (ab247549)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Calpain small subunit 1 antibody [EPR3323] - BSA and Azide free
See all Calpain small subunit 1 primary antibodies -
Description
Rabbit monoclonal [EPR3323] to Calpain small subunit 1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Detects Calpain small subunit 1.
-
Tested applications
Suitable for: ICC, Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Calpain small subunit 1 aa 50-150. The exact sequence is proprietary.
Database link: P04632 -
General notes
Ab247549 is the carrier-free version of ab92356. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3323 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of a HRP/AP polymerized secondary antibody is recommended.
|
|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
Target
-
Function
Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Sequence similarities
Contains 5 EF-hand domains. -
Domain
The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding. - Information by UniProt
-
Database links
- Entrez Gene: 826 Human
- Entrez Gene: 12336 Mouse
- Entrez Gene: 29156 Rat
- Omim: 114170 Human
- SwissProt: P04632 Human
- SwissProt: O88456 Mouse
- SwissProt: Q64537 Rat
- Unigene: 515371 Human
see all -
Alternative names
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
Datasheets and documents
References
ab247549 has not yet been referenced specifically in any publications.
