Recombinant
RabMAb

Anti-Calpain small subunit 1 antibody [SP81] (ab101683)

Overview

  • Product name
    Anti-Calpain small subunit 1 antibody [SP81]
    See all Calpain small subunit 1 primary antibodies
  • Description
    Rabbit monoclonal [SP81] to Calpain small subunit 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calpain small subunit 1 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P04632

  • Positive control
    • FC: HeLa, PC-12 and NIH/3T3 IHC-P: Human breast carcinoma
  • General notes

Properties

Applications

Our Abpromise guarantee covers the use of ab101683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Antigen retrieval is recommeded: boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt 1/20.

Target

  • Function
    Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Sequence similarities
    Contains 5 EF-hand domains.
  • Domain
    The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
    EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30K antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase small subunit antibody
    • Calcium-dependent protease small subunit 1 antibody
    • Calcium-dependent protease small subunit antibody
    • Calpain 4 antibody
    • Calpain 4 small subunit (30K) antibody
    • Calpain 4 small subunit antibody
    • Calpain regulatory subunit antibody
    • Calpain S1 antibody
    • Calpain small polypeptide antibody
    • Calpain small subunit 1 antibody
    • Calpain4 antibody
    • CalpainS1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANPS antibody
    • CAPN 4 antibody
    • CAPN S1 antibody
    • CAPN4 antibody
    • CAPNS 1 antibody
    • CAPNS antibody
    • Capns1 antibody
    • CDPS antibody
    • CPNS1_HUMAN antibody
    • CSS 1 antibody
    • CSS1 antibody
    see all

Images

  • Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemical analysis of Calpain expression in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101683 at 1/100 dilution.
  • Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

References

ab101683 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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