Anti-Calpastatin antibody (ab226249)
Key features and details
- Rabbit polyclonal to Calpastatin
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Calpastatin antibody
See all Calpastatin primary antibodies -
Description
Rabbit polyclonal to Calpastatin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide within Human Calpastatin aa 600-650. The exact sequence is proprietary.
Database link: P20810 -
Positive control
- WB: HeLa anf HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226249 was affinity purified using an epitope specific to Calpastatin immobilized on solid support. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 77 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. -
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family. -
Domain
Has four inhibitory domains. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 462348 Chimpanzee
- Entrez Gene: 281039 Cow
- Entrez Gene: 479148 Dog
- Entrez Gene: 831 Human
- Entrez Gene: 12380 Mouse
- Entrez Gene: 100189891 Orangutan
- Entrez Gene: 397135 Pig
- Entrez Gene: 25403 Rat
see all -
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
see all
Images
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Western blot - Anti-Calpastatin antibody (ab226249)All lanes : Anti-Calpastatin antibody (ab226249) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Calpastatin antibody (ab226249)Calpastatin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226249 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-Calpastatin antibody at 1 µg/ml.
Lane 1: ab226249 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab226249 has not yet been referenced specifically in any publications.
