Product nameAnti-Calpastatin antibody
See all Calpastatin primary antibodies
DescriptionRabbit polyclonal to Calpastatin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within Human Calpastatin aa 600-650. The exact sequence is proprietary.
Database link: P20810
- WB: HeLa anf HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226249 was affinity purified using an epitope specific to Calpastatin immobilized on solid support.
Our Abpromise guarantee covers the use of ab226249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSpecific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similaritiesBelongs to the protease inhibitor I27 (calpastatin) family.
DomainHas four inhibitory domains.
modificationsThe N-terminus is blocked.
- Information by UniProt
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
All lanes : Anti-Calpastatin antibody (ab226249) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Calpastatin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226249 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-Calpastatin antibody at 1 µg/ml.
Lane 1: ab226249 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab226249 has not yet been referenced specifically in any publications.