Product nameAnti-Calpastatin antibody
See all Calpastatin primary antibodies
DescriptionRabbit polyclonal to Calpastatin
Specificityab28252 recognises Calpastatin aminoterminal end (Domain L) testis isoform
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide to the amino terminal end of the testis isoform of human calpstatin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric detection system (BCIP/NBT substrates); 1/5000 when using chemoluminescent substrates. Predicted molecular weight: 76 kDa. But note that when used against the reduced protein identifies bands at 70 Kd, 52 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionSpecific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similaritiesBelongs to the protease inhibitor I27 (calpastatin) family.
DomainHas four inhibitory domains.
modificationsThe N-terminus is blocked.
- Information by UniProt
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Calpastatin antibody (ab28252) at 1/5000 dilution
All lanes : Mouse skeletal tissue muscle lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated Goat IgG to Rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 25,35,74 kDa why is the actual band size different from the predicted?
Lanes 1-3 are skeletal muscle samples from different mice.
This product has been referenced in:
- Zeng X et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). Read more (PubMed: 29069832) »
- Kemp CM et al. The effects of Capn1 gene inactivation on skeletal muscle growth, development, and atrophy, and the compensatory role of other proteolytic systems. J Anim Sci 91:3155-67 (2013). Read more (PubMed: 23798514) »