Key features and details
- Rabbit polyclonal to Calponin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calponin 1 antibody
See all Calponin 1 primary antibodies
DescriptionRabbit polyclonal to Calponin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Recombinant Human Calponin protein (ab114213) can be used as a positive control in WB. This antibody gave a positive signal in the following Human tissue lysates: Small Intestine; Heart; Bladder; Colon.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab78491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).|
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similaritiesBelongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
All lanes : Anti-Calponin 1 antibody (ab78491) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
IHC image of ab78491 staining in Human Normal Breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78491, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab78491 has not yet been referenced specifically in any publications.