Overview

  • Product name

    Anti-Calponin 1 antibody [CALP]
    See all Calponin 1 primary antibodies
  • Description

    Mouse monoclonal [CALP] to Calponin 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Human Calponin 1.

  • Positive control

    • Myoepithelium in normal breast glands.

Properties

Applications

Our Abpromise guarantee covers the use of ab700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/400.

ABC system. 30mins at RT.Calponin 1 has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin 1 has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.

Flow Cyt Use at an assay dependent concentration. PubMed: 20618694

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20138661
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.

Target

  • Function

    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificity

    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • Sequence similarities

    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Images

  • ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor® 488 conjugated goat anti-mouse antibody was used as the secondary. The image was taken  with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin 1 (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin 1 staining in the arterial smooth muscle in the heart tissue with very little background.

    See Abreview

  • ab700 staining Calponin 1 in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).

    See Abreview

References

This product has been referenced in:

  • Fang L  et al. Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells via interaction with tropoelastin mRNA. Int J Mol Med 43:1597-1610 (2019). Read more (PubMed: 30720050) »
  • Kasprzyk-Pawelec A  et al. microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples. Adv Med Sci 64:331-337 (2019). Read more (PubMed: 31022558) »
See all 19 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human arterial myocytes)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Human arterial myocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 08 2015

Answer

The first reference on the datasheet contains the only western blot I have found for this clone, in Figure 8, but it is cropped.

Wu W et al. Artificial niche combining elastomeric substrate and platelets guides vascular differentiation of bone marrow mononuclear cells. Tissue Eng Part A 17:1979-92 (2011).

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142637/

The earliest references we have for this clone, from 1999, appear to characterize the antibody only by IHC. I do not have access to those journals to confirm.

Have you considered the rabbit monoclonal ab46794? The western blot suggests good specificity but as far as we know, it has never been tested in flow cytometry, only ICC and a few other applications.

https://www.abcam.com/index.html?datasheet=700

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Smooth muscle cells from mesenteric artery)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Smooth muscle cells from mesenteric artery
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 17 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart tissue)
Antigen retrieval step
Heat mediated
Specification
Heart tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Fixative
Formaldehyde

Dr. Mal Niladri

Verified customer

Submitted Oct 16 2006

Answer

As far as we are aware, this antibody detects all three.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up