Anti-Calponin 1 antibody [CNN1/832 + CALP] (ab233854)
Key features and details
- Mouse monoclonal [CNN1/832 + CALP] to Calponin 1
- Suitable for: ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Calponin 1 antibody [CNN1/832 + CALP]
See all Calponin 1 primary antibodies -
Description
Mouse monoclonal [CNN1/832 + CALP] to Calponin 1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Human Calponin 1 aa 1 to the C-terminus. (CNN1/832).
Database link: P51911
Tissue, cells or virus. (Crude human uterus extract (CALP)). -
Positive control
- IHC-P: Human uterus and breast carcinoma tissues; Rat uterus tissues. ICC: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
CNN1/832 + CALP -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
Notes |
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ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 1264 Human
- Entrez Gene: 65204 Rat
- Omim: 600806 Human
- SwissProt: P51911 Human
- SwissProt: Q08290 Rat
- Unigene: 465929 Human
- Unigene: 31788 Rat
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Alternative names
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
Images
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Formalin-fixed, paraffin-embedded human uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
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Immunocytochemistry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Calponin 1 with ab233854 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233854 has been referenced in 1 publication.
- Huang CW et al. Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve. Stem Cell Res Ther 10:234 (2019). PubMed: 31376835