Key features and details
- Mouse monoclonal [CNN1/832 + CALP] to Calponin 1
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-Calponin 1 antibody [CNN1/832 + CALP]
See all Calponin 1 primary antibodies
DescriptionMouse monoclonal [CNN1/832 + CALP] to Calponin 1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Human Calponin 1 aa 1-297. (CNN1/832).
MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNF MDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGV KPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQER KFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLD QATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSN KGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA
Database link: P51911
Tissue, cells or virus. (Crude human uterus extract (CALP)).
- IHC-P: Human uterus and breast carcinoma tissues; Rat uterus tissues.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Clone numberCNN1/832 + CALP
Light chain typekappa
Our Abpromise guarantee covers the use of ab233854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similaritiesBelongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
Formalin-fixed, paraffin-embedded human uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233854 has been referenced in 1 publication.
- Huang CW et al. Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve. Stem Cell Res Ther 10:234 (2019). PubMed: 31376835