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    calponin-1-antibody-ep798y-ab46794.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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RecombinantRabMAb

Recombinant Anti-Calponin 1 antibody [EP798Y] (ab46794)

  • Datasheet
  • SDS
Reviews (20)Q&A (7)References (206)

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Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • OI-RD Scanning - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
  • Anti-Calponin 1 antibody [EP798Y] (ab46794)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP798Y] to Calponin 1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Calponin 1 antibody [EP798Y]
    See all Calponin 1 primary antibodies
  • Description

    Rabbit monoclonal [EP798Y] to Calponin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide within Human Calponin 1 aa 250-350 (C terminal). The exact sequence is proprietary.
    Database link: P51911

  • Positive control

    • ICC/IF: C2C12 and HeLa cells; Pig and mouse aortic smooth muscle cells. IHC-P: Rat lung tissue; Mouse cardiac muscle tissue; Human lung carcinoma, kidney, lung, tonsil, uterus, smooth muscle and skeletal muscle tissues. WB: Human bladder lysates, Pig heart lysates, Mouse bladder lysates and Rat bladder lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.73 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP798Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Calponin 1 antibody [EP798Y] (ab197640)
    • Anti-Calponin 1 antibody [EP798Y] - BSA and Azide free (ab216651)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Calponin 1 protein (ab128457)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab46794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
1/5000. Detects a band of approximately 34 kDa.

For unpurified use at 1/20,000.

IHC-P (3)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

ICC/IF (4)
1/500.

For unpurified use at 1/100 - 1/250. 

Notes
WB
1/5000. Detects a band of approximately 34 kDa.

For unpurified use at 1/20,000.

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

ICC/IF
1/500.

For unpurified use at 1/100 - 1/250. 

Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificity

    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • Sequence similarities

    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Target information above from: UniProt accession P51911 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1264 Human
    • Entrez Gene: 12797 Mouse
    • Entrez Gene: 396911 Pig
    • Entrez Gene: 65204 Rat
    • Entrez Gene: 443517 Sheep
    • Omim: 600806 Human
    • SwissProt: P51911 Human
    • SwissProt: Q08091 Mouse
    • SwissProt: Q08092 Pig
    • SwissProt: Q08290 Rat
    • SwissProt: Q7YRL2 Sheep
    • Unigene: 465929 Human
    • Unigene: 4356 Mouse
    • Unigene: 31788 Rat
    see all
  • Alternative names

    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Immunocytochemistry/ Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Calponin 1 with purified ab46794 at 1/500 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/50000 dilution (purified)

    Lane 1 : Human bladder lysates
    Lane 2 : Mouse bladder lysates
    Lane 3 : Rat bladder lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution


    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Calponin 1 with purified ab46794 at a 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.

    PBS instead of the primary antibody was used as the negative control (inset).

  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)Torrado M et al. Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart. PLoS One 6:e26392 (2011). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
    All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Lanes 1-2 : Non-Failing pig LV-myocardium tissue lysate.
    Lanes 3-4 : Failing pig LV-myocardium tissue lysate injected with DOX.


    Ab46794 was used to stain Calponin 1 in Neonatal piglets injected with Dox (Failing LV myocardium) and PBS (Non-Failing LV myocardium). At the protein level a more than twofold increase in Calponin 1 was observed in Dox-injected animals compared to controls (PBS).

  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Paraformadehyde-fixed, 0.25% Triton X-100 permeabilized mouse thoracic aortic smooth muscle cells labeling Calponin 1 using ab46794 at 1/100 dilution in ICC/IF, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150077) at 1/400 dilution.

    1.5% BSA used used as blocking agent for 30 minutes at 25°C. Incubated with primary antibody for 24 hours at 4°C.

    VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells. After fixation, VSMCs were treated with 0.25% Triton X-100 for 20 minutes.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.

    PBS instead of the primary antibody was used as the negative control (inset).

  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/5000 dilution (purified) + Pig heart lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody.

    PBS instead of the primary antibody was used as the negative control (inset).

  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)Image kindly supplied by Mrs Jordan Carbary through Abreview

    Unpurified ab46794 staining Calponin 1 in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence.

    The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)Herington et al PLoS One. 2015 Nov 24;10(11):e0143243. doi: 10.1371/journal.pone.0143243. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Representative photomicrograph of mouse UT-myo cells (Left panel) and murine uterine myometrium (Right panel) stained with smooth muscle cell markers, alpha-SMA (red) and ab46794 (green) and DAPI (blue). UT-myo cells and whole-mount uterine tissue were collected from day 19 of mouse pregnancy. The placenta and embryo were removed from whole-mount tissue sections.

    For full details please see paper.

  • OI-RD Scanning - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    OI-RD Scanning - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified) + Human bladder lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Observed band size: 34 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    ICC/IF image of unpurified ab46794 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    Cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Immunohistochemical staining of paraffin-embedded human smooth muscle using unpurified ab46794 at 1/100 dilution

  • Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified)

    All lanes : Whole tissue lysate prepared from bovine lymph vessels

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 34 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Unpurified ab46794 showing positive staining in normal lung vessel tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Unpurified ab46794 showing positive staining in normal kidney vessels tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Unpurified ab46794 showing positive staining in normal tonsil vessel tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Unpurified ab46794 showing positive staining in normal uterus tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)

    Unpurified ab46794 showing negative staining in skeletal muscle tissue.

  • Anti-Calponin 1 antibody [EP798Y] (ab46794)
    Anti-Calponin 1 antibody [EP798Y] (ab46794)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (206)

Publishing research using ab46794? Please let us know so that we can cite the reference in this datasheet.

ab46794 has been referenced in 206 publications.

  • Wang ZY  et al. Protein deglycase DJ-1 deficiency induces phenotypic switching in vascular smooth muscle cells and exacerbates atherosclerotic plaque instability. J Cell Mol Med N/A:N/A (2021). PubMed: 33501750
  • Zhao G  et al. KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunction. JCI Insight 6:N/A (2021). PubMed: 33507881
  • Yu PK  et al. Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique. Transl Vis Sci Technol 10:29 (2021). PubMed: 33520424
  • Chen H  et al. MicroRNA-124-3p affects myogenic differentiation of adipose-derived stem cells by targeting Caveolin-1 during pelvic floor dysfunction in Sprague Dawley rats. Ann Transl Med 9:161 (2021). PubMed: 33569463
  • Liang Q  et al. Lymphatic muscle cells contribute to dysfunction of the synovial lymphatic system in inflammatory arthritis in mice. Arthritis Res Ther 23:58 (2021). PubMed: 33602317
View all Publications for this product

Customer reviews and Q&As

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1-10 of 20 Abreviews

Immunocytochemistry abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (thoracic aortic smooth muscle cells)
Permeabilization
Yes - 0.25% TritonX-100
Specification
thoracic aortic smooth muscle cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Dr. Mengcheng Shen

Verified customer

Submitted Dec 15 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic kidney)
Permeabilization
Yes - methanol
Specification
embryonic kidney
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 18 2019

Immunocytochemistry abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (human aortic SMCs)
Permeabilization
Yes - 0.1% Triton-X100
Specification
human aortic SMCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 09 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Artery)
Permeabilization
Yes - 0.1% Triton X-100, 10 min
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

DR. Chelsea Stowell

Verified customer

Submitted Mar 07 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Smooth muscle cells)
Permeabilization
Yes - 0.15% Triton-X-100
Specification
Smooth muscle cells
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 05 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calponin 1 antibody [EP798Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted Dec 06 2017

Western blot abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Sep 22 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
Yes - TBST-5 min
Specification
Breast
Blocking step
(agent) for 10 minute(s) · Concentration: 0%
Fixative
Formaldehyde
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Verified customer

Submitted Jun 10 2016

Western blot abreview for Anti-Calponin 1 antibody [EP798Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

Submitted Jul 07 2014

Western blot abreview for Anti-Calponin 1 antibody [EP798Y]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVECells and 5ng/ml TNF-a for 24hr)
Gel Running Conditions
Reduced Denaturing (11% SDS-PAGE)
Loading amount
30 µg
Specification
HUVECells and 5ng/ml TNF-a for 24hr
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Aug 10 2013

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