Overview

  • Product name

    Anti-Calponin 1 antibody [EP798Y] - BSA and Azide free
    See all Calponin 1 primary antibodies
  • Description

    Rabbit monoclonal [EP798Y] to Calponin 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, Pig
  • Immunogen

    aa 250-350 (C terminal). Synthetic peptide corresponding to residues near the C-term of Calponin (Human).

  • Positive control

    • Human bladder lysate; HeLa cells; Human smooth muscle tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
    • Tissue specificity

      Smooth muscle, and tissues containing significant amounts of smooth muscle.
    • Sequence similarities

      Belongs to the calponin family.
      Contains 3 calponin-like repeats.
      Contains 1 CH (calponin-homology) domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • Basic calponin antibody
      • Calponin 1 antibody
      • Calponin 1 basic smooth muscle antibody
      • Calponin H1 antibody
      • Calponin H1 smooth muscle antibody
      • Calponin-1 antibody
      • Calponin1 antibody
      • Calponins basic antibody
      • CNN 1 antibody
      • Cnn1 antibody
      • CNN1_HUMAN antibody
      • Epididymis secretory protein Li 14 antibody
      • HEL S 14 antibody
      • Sm Calp antibody
      • SMCC antibody
      • smooth muscle antibody
      see all

    Images

    • Paraformadehyde-fixed, 0.25% Triton X-100 permeabilized mouse thoracic aortic smooth muscle cells labeling Calponin 1 using ab46794 at 1/100 dilution in ICC/IF, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150077) at 1/400 dilution.

      1.5% BSA used used as blocking agent for 30 minutes at 25°C. Incubated with primary antibody for 24 hours at 4°C.

      VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells. After fixation, VSMCs were treated with 0.25% Triton X-100 for 20 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Representative photomicrograph of UT-myo cells (Left panel) and uterine myometrium (Right panel) stained with smooth muscle cell markers, alpha-SMA (red) and ab46794 (green) and DAPI (blue). UT-myo cells and whole-mount uterine tissue were collected from day 19 of mouse pregnancy. The placenta and embryo were removed from whole-mount tissue sections.

      For full details please see paper.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Immunocytochemistry/ Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Calponin 1 with purified ab46794 at 1:500 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody.

      PBS instead of the primary antibody was used as the negative control (inset).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.

      PBS instead of the primary antibody was used as the negative control (inset).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Calponin 1 with purified ab46794 at a 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.

      PBS instead of the primary antibody was used as the negative control (inset).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Immunohistochemical staining of paraffin-embedded human smooth muscle using unpurified ab46794 at 1/100 dilution

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 staining Calponin in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence.

      The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • ICC/IF image of unpurified ab46794 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      Cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 showing positive staining in normal kidney vessels tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 showing positive staining in normal lung vessel tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 showing positive staining in normal tonsil vessel tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 showing positive staining in normal uterus tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Unpurified ab46794 showing negative staining in skeletal muscle tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab46794).

    References

    This product has been referenced in:

    • Devarasetty M  et al. Bioengineered Submucosal Organoids for In Vitro Modeling of Colorectal Cancer. Tissue Eng Part A 23:1026-1041 (2017). Read more (PubMed: 28922975) »
    • Krawczyk KK  et al. Assessing the contribution of thrombospondin-4 induction and ATF6a activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep 6:32449 (2016). WB ; Mouse . Read more (PubMed: 27581066) »
    See all 38 Publications for this product

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