Overview

  • Product name

    Anti-Calponin 1 antibody [SP180] - C-terminal
    See all Calponin 1 primary antibodies
  • Description

    Rabbit monoclonal [SP180] to Calponin 1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide within Human Calponin 1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P51911

  • Positive control

    • IHC-P: Human colon, breast, kidney, prostate, and tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 10 minutes at room temperature.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Calponin 1 with ab227660 at 1/100 dilution (1.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Calponin 1 with ab227660 at 1/100 dilution (1.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human colon tissue stained for Calponin 1 using ab227660 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for Calponin 1 using ab227660 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for Calponin 1 using ab227660 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Calponin 1 using ab227660 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Calponin 1 using ab227660 at 1/100 dilution in immunohistochemical analysis.

References

ab227660 has not yet been referenced specifically in any publications.

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