Recombinant Anti-Calponin 1 antibody [SP181] (ab227661)
![Western blot - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290522-anti-calponin-1-antibody-sp181-western-blot.jpg "Western blot - Anti-Calponin 1 antibody [SP181] (ab227661)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP181] to Calponin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Calponin 1 antibody [SP181]
See all Calponin 1 primary antibodies -
Description
Rabbit monoclonal [SP181] to Calponin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human bladder lysate. IHC-P: Human colon, breast, kidney, prostate and tonsil tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP181 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
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| WB |
1/125. Predicted molecular weight: 33 kDa.
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| Notes |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
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WB
1/125. Predicted molecular weight: 33 kDa. |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 396522 Chicken
- Entrez Gene: 534583 Cow
- Entrez Gene: 1264 Human
- Entrez Gene: 12797 Mouse
- Entrez Gene: 396911 Pig
- Entrez Gene: 65204 Rat
- Omim: 600806 Human
- SwissProt: P26932 Chicken
see all -
Alternative names
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
Images
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Western blot - Anti-Calponin 1 antibody [SP181] (ab227661)Anti-Calponin 1 antibody [SP181] (ab227661) at 1/125 dilution + Human bladder lysate
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290521-anti-calponin-1-antibody-sp181-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)Formalin-fixed, paraffin-embedded human colon tissue stained for Calponin 1 using ab227661 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290520-anti-calponin-1-antibody-sp181-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)Formalin-fixed, paraffin-embedded human breast tissue stained for Calponin 1 using ab227661 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290519-anti-calponin-1-antibody-sp181-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)Formalin-fixed, paraffin-embedded human kidney tissue stained for Calponin 1 using ab227661 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290518-anti-calponin-1-antibody-sp181-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)Formalin-fixed, paraffin-embedded human prostate tissue stained for Calponin 1 using ab227661 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-290517-anti-calponin-1-antibody-sp181-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP181] (ab227661)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Calponin 1 using ab227661 at 1/100 dilution in immunohistochemical analysis.
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![Anti-Calponin 1 antibody [SP181] (ab227661)](https://www.abcam.com/ps/products/227/ab227661/Images/ab227661-7-benefits-of-recombinant-antibodies.png)
Anti-Calponin 1 antibody [SP181] (ab227661)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab227661 has not yet been referenced specifically in any publications.
