Anti-Calponin 2 antibody (ab129331)
Key features and details
- Goat polyclonal to Calponin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calponin 2 antibody -
Description
Goat polyclonal to Calponin 2 -
Host species
Goat -
Specificity
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Calponin 2 aa 292-306 (internal sequence). Near the C terminus of Human Calponin 2 (NP_004359.1).
Sequence:C-DPGEVPEYPPYYQEE
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Positive control
- Human Placenta tissue; HepG2 lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Heart and smooth muscle. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 1265 Human
- Omim: 602373 Human
- SwissProt: Q99439 Human
- Unigene: 651512 Human
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Alternative names
- Calponin 2 antibody
- Calponin H2 antibody
- Calponin H2, smooth muscle antibody
see all
Images
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ab129331, at 2.5 µg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
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Anti-Calponin 2 antibody (ab129331) at 0.03 µg/ml + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab129331 has not yet been referenced specifically in any publications.