Overview

  • Product name

  • Description

    Goat polyclonal to Calponin 2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Calponin 2 aa 292-306 (internal sequence). Near the C terminus of Human Calponin 2 (NP_004359.1).
    Sequence:

    C-DPGEVPEYPPYYQEE

  • Positive control

    • Human Placenta tissue; HepG2 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab129331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificity

    Heart and smooth muscle.
  • Sequence similarities

    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calponin 2 antibody
    • Calponin H2 antibody
    • Calponin H2, smooth muscle antibody
    • Calponin-2 antibody
    • Cnn2 antibody
    • CNN2_HUMAN antibody
    • Neutral calponin antibody
    • smooth muscle antibody
    see all

Images

  • ab129331, at 2.5 µg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.

  • Anti-Calponin 2 antibody (ab129331) at 0.03 µg/ml + HepG2 lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    Primary incubation was 1 hour.

References

ab129331 has not yet been referenced specifically in any publications.

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