Key features and details
- Goat polyclonal to Calponin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calponin 2 antibody
See all Calponin 2 primary antibodies
DescriptionGoat polyclonal to Calponin 2
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Gorilla, Orangutan
- Human Placenta tissue; HepG2 lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificityHeart and smooth muscle.
Sequence similaritiesBelongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Calponin 2 antibody
- Calponin H2 antibody
- Calponin H2, smooth muscle antibody
ab129331, at 2.5 µg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
Anti-Calponin 2 antibody (ab129331) at 0.03 µg/ml + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Primary incubation was 1 hour.
ab129331 has not yet been referenced specifically in any publications.