Key features and details
- Rabbit polyclonal to Calponin 2
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Calponin 2 antibody
See all Calponin 2 primary antibodies
DescriptionRabbit polyclonal to Calponin 2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Cow, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human Calponin 2 aa 1-269. EXpressed in E.coli.
MSSTQFNKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIGPDF QKGLKDGTILCTLMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGM NPVDLFEANDLFESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQ ERNFDDATMKAGQCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNHILPP MDHSTISLQMGTNKCASQVGMTAPGTRRHIYDTKLGTDKCDNSSMSLQMG YTQGANQSGQVFGLGRQIY
Database link: Q99439
- WB: Recombinant human Calponin 2 protein; Human lung lysate; Pig lymph node lysate; HeLa and HepG2 cell lysates. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 20 µg/ml. Predicted molecular weight: 34 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificityHeart and smooth muscle.
Sequence similaritiesBelongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Calponin 2 antibody
- Calponin H2 antibody
- Calponin H2, smooth muscle antibody
Anti-Calponin 2 antibody (ab233204) at 2 µg/ml + Recombinant human Calponin 2 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 34 kDa
All lanes : Anti-Calponin 2 antibody (ab233204) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Pig lymph node lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 34 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calponin 2 (green) using ab233204 at 20 μg/ml in ICC/IF. FITC labeling.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233204 has not yet been referenced specifically in any publications.