Anti-Calponin 3 antibody (ab151427)
Key features and details
- Rabbit polyclonal to Calponin 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Calponin 3 antibody
See all Calponin 3 primary antibodies -
Description
Rabbit polyclonal to Calponin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human Calponin 3 aa 68-312.
Database link: Q15417 -
Positive control
- 293T, HeLa, HepG2, NIH 3T3 and JC whole cell lysates; Human U87 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Expressed in both non-smooth muscle tissues as well as smooth muscle tissues. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
-
Database links
- Entrez Gene: 538975 Cow
- Entrez Gene: 1266 Human
- Entrez Gene: 71994 Mouse
- Entrez Gene: 54321 Rat
- Entrez Gene: 380174 Xenopus laevis
- Entrez Gene: 394869 Xenopus tropicalis
- Omim: 602374 Human
- SwissProt: Q32L92 Cow
see all -
Alternative names
- Acidic calponin 3 antibody
- acidic isoform antibody
- Calponin 3 acidic antibody
see all
Images
-
All lanes : Anti-Calponin 3 antibody (ab151427) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS-PAGE. -
All lanes : Anti-Calponin 3 antibody (ab151427) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS-PAGE. -
Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling Calponin 3 with ab151427 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab151427 has been referenced in 2 publications.
- She Y et al. Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway. Front Physiol 12:659272 (2021). PubMed: 34305633
- Dai F et al. Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma. Aging (Albany NY) 12:14037-14049 (2020). PubMed: 32667904