Overview

  • Product name

  • Description

    Rabbit polyclonal to Calponin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human Calponin 3 aa 68-312.
    Database link: Q15417

  • Positive control

    • 293T, HeLa, HepG2, NIH 3T3 and JC whole cell lysates; Human U87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-Calponin 3 antibody (ab151427) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE.
  • All lanes : Anti-Calponin 3 antibody (ab151427) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS-PAGE.
  • Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling Calponin 3 with ab151427 at 1/500 dilution.

References

ab151427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151427.
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