Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)
Key features and details
- Mouse monoclonal [CNN1/832 + CALP] to Calponin 1 - BSA and Azide free
- Suitable for: IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free
See all Calponin 1 primary antibodies -
Description
Mouse monoclonal [CNN1/832 + CALP] to Calponin 1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Human Calponin 1 aa 1 to the C-terminus. (CNN1/832).
Database link: P51911
Tissue, cells or virus corresponding to Human Calponin 1. (Crude human uterus extract (CALP)). -
Positive control
- Human uterus and breast carcinoma tissues; Rat uterus tissue. ICC; K562 cells
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General notes
CNN1/832: IgG1 kappa. CALP: IgG1 kappa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab212306 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
CNN1/832 + CALP -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
|
ICC |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 1264 Human
- Entrez Gene: 65204 Rat
- Omim: 600806 Human
- SwissProt: P51911 Human
- SwissProt: Q08290 Rat
- Unigene: 465929 Human
- Unigene: 31788 Rat
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Alternative names
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat uterus tissue labeling Calponin 1 with ab212306 at 1 µg/ml.
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Immunocytochemistry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Calponin 1 with ab212306 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling Calponin 1 with ab212306 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling Calponin 1 with ab212306 at 1 µg/ml.
Datasheets and documents
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Datasheet download
References (0)
ab212306 has not yet been referenced specifically in any publications.