Anti-Calponin antibody - N-terminal (ab175572)

Overview

  • Product name
    Anti-Calponin antibody - N-terminal
    See all Calponin primary antibodies
  • Description
    Rabbit polyclonal to Calponin - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human Calponin aa 17-47 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P51911

  • Positive control
    • NCI-H460 cell line lysates; Human lung carcinoma tissue; NCI-H292 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 33 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificity
    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • Sequence similarities
    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Calponin with ab175572 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-Calponin antibody - N-terminal (ab175572) at 1/1000 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size: 33 kDa

  • Flow Cytometric analysis of NCI-H292 cells labeling Calponin with ab175572 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (aortic smooth muscle cells)
Permeabilization
Yes - 0,2% Tween-20 in PBS
Gating Strategy
marker expression
Specification
aortic smooth muscle cells
Preparation
Cell harvesting/tissue preparation method: Trypsin/EDTA
Sample buffer: 0,3%FCS in PBS
Fixation
none
Username

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Verified customer

Submitted Nov 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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