Anti-Calreticulin antibody (ab15607)
Key features and details
- Rabbit polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Calreticulin antibody
See all Calreticulin primary antibodies -
Description
Rabbit polyclonal to Calreticulin -
Host species
Rabbit -
Specificity
This antibody is specific for Calreticulin. It does not recognise other ER proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide:
QAKDEL
, corresponding to C terminal amino acids 412-417 of Human Calreticulin. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using BSA-QAKKDEL attached to CNBr beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 60 kDa.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa. |
Target
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Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 12317 Mouse
- SwissProt: P14211 Mouse
- Unigene: 1971 Mouse
- Unigene: 467043 Mouse
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Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab15607 has been referenced in 1 publication.
- Saavedra MD et al. Calreticulin from suboolemmal vesicles affects membrane regulation of polyspermy. Reproduction 147:369-78 (2014). WB ; Pig . PubMed: 24398873