Overview

  • Product name

    Anti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description

    Rabbit polyclonal to Calreticulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Calreticulin conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Database link: P27797

  • Positive control

    • WB: A431, A549, HCT 116, HeLa, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T89G cell lysates. ICC/IF: Heat shocked HeLa cells. IHC-P: Mouse backskin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100.
IHC-P 1/100.

Target

  • Function

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities

    Belongs to the calreticulin family.
  • Domain

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Images

  • All lanes : Anti-Calreticulin antibody (ab223614) at 1/5000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
    Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 12 : T98G cell lysate
    Lane 13 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG HRP

    Predicted band size: 48 kDa



    Blocking buffer: 1.5% BSA.

    Primary and secondary antibodies were incubated for 2 hours at RT.

  • 2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (yellow) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

    (A) DAPI nuclear stain (B) ab223614 (C) Merge.

     

  • 2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (green) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

    (A) DAPI nuclear stain (B) ab223614 (C) Merge.

  • Bouin's-fixed mouse backskin stained for Calreticulin (green) using ab223614 at 1/100 dilution in immunohistochemical analysis. Secondary: Goat anti-Rabbit FITC at 1/50 dilution for 1 hour at RT.

References

ab223614 has not yet been referenced specifically in any publications.

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