Key features and details
- Rabbit polyclonal to Calreticulin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Calreticulin antibody
See all Calreticulin primary antibodies
DescriptionRabbit polyclonal to Calreticulin
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Calreticulin conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: P27797
- WB: A431, A549, HCT 116, HeLa, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T89G cell lysates. ICC/IF: Heat shocked HeLa cells. IHC-P: Mouse backskin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
- HL60 whole cell lysate (ab7914)
Our Abpromise guarantee covers the use of ab223614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 48 kDa.|
FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Sequence similaritiesBelongs to the calreticulin family.
DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
All lanes : Anti-Calreticulin antibody (ab223614) at 1/5000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
All lanes : Donkey Anti-Rabbit IgG HRP
Predicted band size: 48 kDa
Blocking buffer: 1.5% BSA.
Primary and secondary antibodies were incubated for 2 hours at RT.
2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (yellow) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223614 (C) Merge.
2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (green) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223614 (C) Merge.
Bouin's-fixed mouse backskin stained for Calreticulin (green) using ab223614 at 1/100 dilution in immunohistochemical analysis. Secondary: Goat anti-Rabbit FITC at 1/50 dilution for 1 hour at RT.
ab223614 has been referenced in 1 publication.
- Jiang Z et al. Calreticulin Blockade Attenuates Murine Acute Lung Injury by Inducing Polarization of M2 Subtype Macrophages. Front Immunol 11:11 (2020). PubMed: 32082309