Anti-Calreticulin antibody (ab265598)
Key features and details
- Rabbit polyclonal to Calreticulin
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Calreticulin antibody
See all Calreticulin primary antibodies -
Description
Rabbit polyclonal to Calreticulin -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Calreticulin aa 367-417. The exact sequence is proprietary. NP_004334.1
Database link: P27797 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab265598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-5 µg/mg of lysate.
|
|
WB |
1/2000 - 1/10000. Predicted molecular weight: 48 kDa.
|
Notes |
---|
IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
-
Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
-
Database links
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Omim: 109091 Human
- SwissProt: P27797 Human
- SwissProt: P14211 Mouse
- Unigene: 515162 Human
- Unigene: 1971 Mouse
- Unigene: 467043 Mouse
-
Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
-
All lanes : Anti-Calreticulin antibody (ab265598) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 48 kDa
Exposure time: 1 second -
Calreticulin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265598 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265598 at 0.1 µg/ml.
Lane 1: ab265598 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab265598 has not yet been referenced specifically in any publications.