Anti-Calreticulin antibody (ab2908)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Calreticulin antibody
See all Calreticulin primary antibodies -
DescriptionChicken polyclonal to Calreticulin
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Host speciesChicken
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Tested applicationsSuitable for: WB, ICC, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human, Non human primates
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Immunogen
Synthetic peptide corresponding to Mouse Calreticulin aa 399-414.
Sequence:DEKEEDEEESPGQAKD
(Peptide available asab4927) -
Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Immunizing Peptide (Blocking)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Can be blocked with Calreticulin peptide (ab4927). | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
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Sequence similaritiesBelongs to the calreticulin family.
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DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
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Database links
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 100009050 Rabbit
- Entrez Gene: 64202 Rat
- Omim: 109091 Human
- SwissProt: P27797 Human
- SwissProt: P14211 Mouse
- SwissProt: P15253 Rabbit
see all -
Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
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Western blot - Anti-Calreticulin antibody (ab2908)All lanes : Anti-Calreticulin antibody (ab2908) at 1/2000 dilution
Lane 1 : MCF7
Lane 2 : MDA-MB-231
Lane 3 : HeLa
Lane 4 : K-562
Lane 5 : Jurkat
Lane 6 : A549
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Calreticulin antibody (ab2908)Western blot of calreticulin on HeLa cell extract using ab2908. Western blot of calreticulin on HeLa cell extract using ab2908.
Protocols
References
This product has been referenced in:
- Ahn J et al. Extrinsic Phagocyte-Dependent STING Signaling Dictates the Immunogenicity of Dying Cells. Cancer Cell 33:862-873.e5 (2018). Read more (PubMed: 29706455) »
- Guan P et al. Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-a expression. Exp Ther Med 13:1408-1414 (2017). Read more (PubMed: 28413486) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"