Anti-Calreticulin antibody (ab2908)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Calreticulin antibody
See all Calreticulin primary antibodies -
DescriptionChicken polyclonal to Calreticulin
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Host speciesChicken
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Tested applicationsSuitable for: WB, ICC, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human, Non human primates
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Immunogen
Synthetic peptide corresponding to Mouse Calreticulin aa 399-414.
Sequence:DEKEEDEEESPGQAKD
(Peptide available asab4927) -
Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Can be blocked with Calreticulin peptide (ab4927). | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
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Sequence similaritiesBelongs to the calreticulin family.
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DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
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Database links
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 100009050 Rabbit
- Entrez Gene: 64202 Rat
- Omim: 109091 Human
- SwissProt: P27797 Human
- SwissProt: P14211 Mouse
- SwissProt: P15253 Rabbit
see all -
Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
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Western blot - Anti-Calreticulin antibody (ab2908)All lanes : Anti-Calreticulin antibody (ab2908) at 1/2000 dilution
Lane 1 : MCF7
Lane 2 : MDA-MB-231
Lane 3 : HeLa
Lane 4 : K-562
Lane 5 : Jurkat
Lane 6 : A549
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Calreticulin antibody (ab2908)Western blot of calreticulin on HeLa cell extract using ab2908. Western blot of calreticulin on HeLa cell extract using ab2908.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Ahn J et al. Extrinsic Phagocyte-Dependent STING Signaling Dictates the Immunogenicity of Dying Cells. Cancer Cell 33:862-873.e5 (2018). Read more (PubMed: 29706455) »
- Zhang L et al. The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLoS Pathog 14:e1007435 (2018). Read more (PubMed: 30388174) »
