Overview

  • Product name
    Anti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description
    Chicken polyclonal to Calreticulin
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, ICC, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Mouse Calreticulin aa 399-414.
    Sequence:

    DEKEEDEEESPGQAKD


    (Peptide available as ab4927)

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Can be blocked with Calreticulin peptide (ab4927).
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities
    Belongs to the calreticulin family.
  • Domain
    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization
    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Images

  • All lanes : Anti-Calreticulin antibody (ab2908) at 1/2000 dilution

    Lane 1 : MCF7
    Lane 2 : MDA-MB-231
    Lane 3 : HeLa
    Lane 4 : K-562
    Lane 5 : Jurkat
    Lane 6 : A549

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml

    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

  • Western blot of calreticulin on HeLa cell extract using ab2908. Western blot of calreticulin on HeLa cell extract using ab2908.

References

This product has been referenced in:
  • Ahn J  et al. Extrinsic Phagocyte-Dependent STING Signaling Dictates the Immunogenicity of Dying Cells. Cancer Cell 33:862-873.e5 (2018). Read more (PubMed: 29706455) »
  • Conley SM  et al. Rom1 converts Y141C-Prph2-associated pattern dystrophy to retinitis pigmentosa. Hum Mol Genet 26:509-518 (2017). Read more (PubMed: 28053051) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U-2 OS, A549, ASC/TERT1)
Permeabilization
Yes - 0.1% Triton X-100
Specification
U-2 OS, A549, ASC/TERT1
Fixative
Paraformaldehyde

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Verified customer

Submitted Oct 26 2018

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