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Anti-Calreticulin antibody (ab2908)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Western blot - Anti-Calreticulin antibody (ab2908)
  • Western blot - Anti-Calreticulin antibody (ab2908)

Key features and details

  • Chicken polyclonal to Calreticulin
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgY

You may also be interested in

Protein
Product image
Recombinant Rabbit Calreticulin protein (ab15729)
Secondary
Product image
Goat Anti-Chicken IgY H&L (HRP) (ab6877)

View more associated products

Overview

  • Product name

    Anti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description

    Chicken polyclonal to Calreticulin
  • Host species

    Chicken
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Calreticulin aa 399-414.
    Sequence:

    DEKEEDEEESPGQAKD


    (Peptide available as ab4927)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Rabbit Calreticulin protein (ab15729)
  • Related Products

    • Recombinant Rabbit Calreticulin protein (ab15729)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000.
Notes
WB
1/2000.

Target

  • Function

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities

    Belongs to the calreticulin family.
  • Domain

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Target information above from: UniProt accession P27797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 811 Human
    • Omim: 109091 Human
    • SwissProt: P27797 Human
    • Unigene: 515162 Human
    • Alternative names

      • Autoantigen RO antibody
      • CALR antibody
      • CALR protein antibody
      • CALR_HUMAN antibody
      • Calregulin antibody
      • Calreticulin antibody
      • cC1qR antibody
      • CRP55 antibody
      • CRT antibody
      • CRTC antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • Epididymis secretory sperm binding protein Li 99n antibody
      • ERp60 antibody
      • FLJ26680 antibody
      • grp60 antibody
      • HACBP antibody
      • HEL S 99n antibody
      • RO antibody
      • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
      • Sicca syndrome antigen A antibody
      • SSA antibody
      see all

    Images

    • Western blot - Anti-Calreticulin antibody (ab2908)
      Western blot - Anti-Calreticulin antibody (ab2908)
      All lanes : Anti-Calreticulin antibody (ab2908) at 1/2000 dilution

      Lane 1 : MCF7
      Lane 2 : MDA-MB-231
      Lane 3 : HeLa
      Lane 4 : K-562
      Lane 5 : Jurkat
      Lane 6 : A549

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml

      Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-Calreticulin antibody (ab2908)
      Western blot - Anti-Calreticulin antibody (ab2908)
      Western blot of calreticulin on HeLa cell extract using ab2908. Western blot of calreticulin on HeLa cell extract using ab2908.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab2908? Please let us know so that we can cite the reference in this datasheet.

    ab2908 has been referenced in 4 publications.

    • Ahn J  et al. Extrinsic Phagocyte-Dependent STING Signaling Dictates the Immunogenicity of Dying Cells. Cancer Cell 33:862-873.e5 (2018). PubMed: 29706455
    • Zhang L  et al. The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLoS Pathog 14:e1007435 (2018). PubMed: 30388174
    • Guan P  et al. Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-a expression. Exp Ther Med 13:1408-1414 (2017). PubMed: 28413486
    • Conley SM  et al. Rom1 converts Y141C-Prph2-associated pattern dystrophy to retinitis pigmentosa. Hum Mol Genet 26:509-518 (2017). PubMed: 28053051

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U-2 OS, A549, ASC/TERT1)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    U-2 OS, A549, ASC/TERT1
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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