Anti-Calreticulin antibody (ab2908)
Key features and details
- Chicken polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-Calreticulin antibody
See all Calreticulin primary antibodies -
Description
Chicken polyclonal to Calreticulin -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Mouse Calreticulin aa 399-414.
Sequence:DEKEEDEEESPGQAKD
(Peptide available asab4927) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000.
|
| Notes |
|---|
|
WB
1/2000. |
Target
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Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 811 Human
- Omim: 109091 Human
- SwissProt: P27797 Human
- Unigene: 515162 Human
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Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
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Western blot - Anti-Calreticulin antibody (ab2908)All lanes : Anti-Calreticulin antibody (ab2908) at 1/2000 dilution
Lane 1 : MCF7
Lane 2 : MDA-MB-231
Lane 3 : HeLa
Lane 4 : K-562
Lane 5 : Jurkat
Lane 6 : A549
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Calreticulin antibody (ab2908)Western blot of calreticulin on HeLa cell extract using ab2908. Western blot of calreticulin on HeLa cell extract using ab2908.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab2908 has been referenced in 4 publications.
- Ahn J et al. Extrinsic Phagocyte-Dependent STING Signaling Dictates the Immunogenicity of Dying Cells. Cancer Cell 33:862-873.e5 (2018). PubMed: 29706455
- Zhang L et al. The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLoS Pathog 14:e1007435 (2018). PubMed: 30388174
- Guan P et al. Cigarette smoke extract promotes proliferation of airway smooth muscle cells through suppressing C/EBP-a expression. Exp Ther Med 13:1408-1414 (2017). PubMed: 28413486
- Conley SM et al. Rom1 converts Y141C-Prph2-associated pattern dystrophy to retinitis pigmentosa. Hum Mol Genet 26:509-518 (2017). PubMed: 28053051