Product nameAnti-Calreticulin antibody
See all Calreticulin primary antibodies
DescriptionChicken polyclonal to Calreticulin
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Dog, Human
Predicted to work with: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey
- AtT-20 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab94935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).|
|ICC||Use at an assay dependent concentration.|
FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Sequence similaritiesBelongs to the calreticulin family.
DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution
Lane 1 : A-549
Lane 2 : HEK 293
Lane 3 : Mouse Brain
Lysates/proteins at 30 µg per lane.
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Predicted band size: 48 kDa
Anti-Calreticulin antibody (ab94935) at 1/1000 dilution + AtT-20 cell lysate
Predicted band size: 48 kDa
ab94935 has not yet been referenced specifically in any publications.